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. 2012 Apr 3;22(5):822–835. doi: 10.1038/cr.2012.53

Figure 4.

Figure 4

NLRC5 deficiency increases type I IFN signaling and antiviral immunity in MEFs. (A, B) NLRC5−/− and WT MEFs were infected with VSV-eGFP for the indicated times. Cell lysates were harvested to detect IRF3 phosphorylation by immunoblotting (A) or the RNA was collected to detect the expression of Ifn-α, Ifn-β, Il-6 and Tnf-α by real-time PCR analysis (B). (C) MEFs were treated with poly(I:C)/LyoVec or infected with VSV-eGFP for the indicated times. The culture supernatants were harvested for ELISA analyses to measure the production of IFN-β. (D) MAVS−/−, WT and NLRC5−/− MEFs were infected by VSV-eGFP (MOI = 1) for the indicated times, viral infections were determined by fluorescence microscopy for GFP-positive cells and phase contrast as a control. B and C are plotted as means ± SD. Results are representative of three independent experiments. *P < 0.05, **P < 0.01, versus controls.