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. 2012 Apr 3;22(5):822–835. doi: 10.1038/cr.2012.53

Figure 6.

Figure 6

Enhanced NF-κB and type I IFN signaling in NLRC5−/− BMMs. (A) NLRC5−/− and WT BMMs were treated with LPS for the indicated times, and the cell lysates were harvested for immunoblotting with the indicated antibodies. (B) BMMs were treated with LPS, CL-097 or CpG for the indicated times, and the culture supernatants were harvested for ELISA analysis. (C) NLRC5−/− and WT BMMs were infected by VSV-eGFP for the indicated times. Cell lysates were harvested to detect IRF3 phosphorylation by immunoblotting. (D) NLRC5−/− and WT BMMs were treated with VSV-eGFP, poly(I:C)/LyoVec or poly(I:C) and the culture supernatants were used for ELISA analysis for IFN-β production. B and D are plotted as means ± SD. Results are representative of three independent experiments. *P < 0.05, **P < 0.01, versus controls.