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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Clin Immunol. 2012 Feb 10;143(2):188–199. doi: 10.1016/j.clim.2012.01.019

Figure 3.

Figure 3

Figure 3

Stimulation of signaling pathways by LPS in gingival fibroblasts and U937 cells. A. Gingival fibroblasts or U937 cells were treated with 100 ng/ml of LPS for different times as indicated. At each time point, cellular phosphorylated p44/p42 (phospho-p44/42), JNK (phosphor-JNK), p38 (phosphor-p38) as well as IκB alpha and GAPDH were detected using immunoblotting as described in Experimental Procedure. B. Gingival fibroblasts or U937 cells were treated with 100 ng/ml of LPS for different times as indicated. At each time point, nuclear NFκB p50 and NFκB p65, and cellular GAPDH were detected using immunoblotting as described in Experimental Procedure. The graphs presented are from one of two independent experiments with similar results.