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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Clin Immunol. 2012 Feb 10;143(2):188–199. doi: 10.1016/j.clim.2012.01.019

Figure 5.

Figure 5

The effect of LPS on nuclear activities of AP-1 subunits in U937 cells and gingival fibroblasts. U937 cells (A) or gingival fibroblasts (B) were treated with or without 100 ng/ml of LPS for 2 h. After the treatment, the nuclear proteins were isolated from cells and subjected to a transcription factor activity assay system in which AP-1-binding element-containing oligonucleotides were immobilized on the surface of wells. After incubating the wells with nuclear proteins, the AP-1 subunits bound to the oligonucleotides were identified by specific antibodies. The bound levels of c-Fos, Fos B, Fra-1, Jun B, Jun D and phosphorylated c-Jun (p-c-Jun) were determined. The data presented are representative of two experiments with similar results.