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Cellular localization of WT and mutant αs. HEK-293 cells transiently expressed αs-WT (A and B) or αsΔ (C and D), in the absence (A and C) or presence (B and D) of coexpressed β1γ2. Fixation and immunofluorescent detection of HA epitopes incorporated into the recombinant αs proteins are described in Materials and Methods.
