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Effect of dopamine and NGF withdrawal on nuclear morphology. Neuronal PC12 cells were incubated (24 h) in the presence and absence of NGF or in the presence of NGF plus 300 μM dopamine (DA). The effect of the addition of 0.1 μM clorgyline (Clor) was examined. The cells were fixed, stained with 4,6-diamidino-2-phenylindole, and inspected by fluorescence microscopy. Representative images of PC12 cells are shown. Similar data were obtained in three independent experiments.
