Figure 2.

Northern blot analysis of hPIP1 expression in different human tissues. Multiple Tissue Northern (MTN) blots were prepared from the purified poly(A)⁺ RNA and blotted onto a positively charged nylon membrane (CLONTECH). The amount of RNA on an MTN blot membrane is adjusted so that the β-actin hybridization signal is of comparable intensity in every lane (CLONTECH). RNA was isolated from following human tissues: 1, brain; 2, heart; 3, muscle; 4, colon; 5, thymus; 6, spleen; 7, kidney; 8, liver; 9, small intestine; 10, placenta; 11, lung; 12, peripheral blood leukocytes. The size of hPIP1 cDNA was estimated to be ≈2.4 kb with minor bands at 4.1 kb.