Figure 5.

Regulation of PAK1-induced AP-1 and NF-κB signaling pathways. (A) Overexpression of hPIP1 blocks Cdc42-PAK1-stimulated AP-1 transcriptional activity. COS-7 cells were cotransfected with either empty vector (lane 1) or cDNAs encoding the listed proteins, together with the reporter pAP1-Luc. RU, relative light units. (B) hPIP1 inhibited the activation of NF-κB transcriptional activity by PAK1 or PAK1 plus Cdc42 in the reporter assays. In the control experiment (lane 1), the cells were transfected with pCMV-lacZ plasmid. The results are expressed as the mean of values determined from at least three independent transfection assays.