Figure 2.
(A) Induction of Egr-1 protein in HBECs by transmembrane pressure of 30 cm H2O. Unstimulated cells (N) are compared to cells fixed after 0.5, 1, and 2 h of mechanical stimulation. (B) The relative amounts of Egr-1 protein detected in HBEC lysates by Western blot after the application of 30 cm H2O transmembrane pressure for 30 min, 1 h, 2 h, or 4 h, or after 30 min stimulation with PMA (mean ± SD, n = 5). Densitometry was used to quantify protein levels relative to the unstimulated cells (**, P < 0.005 in comparison to no pressure using ANOVA).