Table 4.
Cytotoxic and hemolytic activity de Erytroxylum caatingae.
| Products/Extracts | Cell Line, IC50 (μg/mL) | EC50 (μg/mL) | ||
|---|---|---|---|---|
| HEp-2 | NCI-H292 | K562 | ||
| 1 | >50 | >50 | >50 | n.t. |
| 2 | >50 | >50 | >50 | n.t. |
| 3 | >50 | 25.76 ± 1.71 | >50 | >2000 |
| 4 | >50 | 25.50 ± 1.08 | >50 | >2000 |
| 5 | 40.59 ± 0.72 | 28.19 ± 2.09 | 13.10 ± 0.63 | >2000 |
| 6 | 34.12 ± 1.01 | 20.37 ± 0.75 | 9.86 ± 0.56 | 403.24 ± 9.64 |
| 7 | 8.25 ± 0.36 | 34.39 ± 1.71 | 11.21 ± 0.46 | 1036.52 ± 35.17 |
| 8 | 31.42 ± 1.01 | 15.79 ± 1.01 | 33.58 ± 1.33 | 510.00 ± 8.60 |
| 9 | >50 | >50 | >50 | n.t. |
| 10 | >50 | >50 | 9.36 ± 0.77 | >250 |
| 11 | >50 | >50 | >50 | n.t. |
| Etoposide | 6.10 ± 0.19 | 2.75 ± 0.10 | 4.48 ± 0.23 | n.t. |
1: Methanol extract of stem of E. caatingae (MEEC); 2: AcOEt:MeOH (40:60); 3: AcOEt:MeOH (80:20); 4: AcOEt:MeOH (90:10); 5: AcOEt:MeOH (95:5); 6: acetate fraction (AcOEt); 7: chloroform fraction (CHCl3); 8: hexane fraction (C6H6); 9: Fraction total of alkaloids; 10: catuabine B; 11: 3α,6β-dibenzoyloxytropane; n.t.: not tested. The IC50 and EC50 and its 95% confidence interval (CI 95%) were obtained by non-linear regression. Data are presented as mean ± SD of two independent experiments. Each experiment was done in triplicate.