Table 1.
ISSR primers used for DNA amplifications, PCR annealing temperature (Ta), number of bands per primer, number and percentage of polymorphic bands.
| Primer code | Primer sequence (5′–3′) | Ta (°C) | No. of bands per primer | No. of polymorphic bands | Polymorphism (%) |
|---|---|---|---|---|---|
| 17 | (AG)8TC | 52 | 10 | 10 | 100.00 |
| 815 | (CT)8G | 52 | 13 | 12 | 92.31 |
| 824 | (TC)8G | 50 | 8 | 7 | 87.50 |
| 834 | (AG)8YT | 52 | 9 | 9 | 100.00 |
| 836 | (AT)8YA | 50 | 7 | 7 | 100.00 |
| 840 | (GA)8YT | 52 | 15 | 15 | 100.00 |
| 846 | (CA)8RT | 52 | 8 | 8 | 100.00 |
| 848 | (CA)8RG | 52 | 15 | 15 | 100.00 |
| 866 | (CTC)5 | 52 | 13 | 13 | 100.00 |
| 895 | AGA GTT GGT AGC TCT TGA TC |
52 | 12 | 10 | 83.33 |
| Total | 110 | 106 | 96.36 |
Y = (C, T); R = (A, G).