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. 2012 Apr 10;13(4):4433–4445. doi: 10.3390/ijms13044433

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© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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(A) Localization of transient receptor potential vanilloid (TRPV) channel isoforms (TRPV4, TRPV5 and TRPV6) in equine articular cartilage. Sections of equine articular cartilage were immunostained using polyclonal antibodies raised against TRPV4, TRPV5 or TRPV6. All three channels showed positive immunoreactivity in chondrocytes from the superficial zone of equine articular cartilage. The negative control shown was treated using the same immunohistochemical protocol, but the primary antibody step was omitted; (B) Cellular and subcellular localization of TRPV4, TRPV5 and TRPV6 in monolayer cultures of methanol fixed P2 chondrocytes. All three channels showed positive immunoreactivity (green fluorescent staining) within P2 chondrocytes. The negative control was exposed to nonimmune rabbit IgG and shows blue fluorescent nuclear staining only. Nuclei were counterstained with DAPI (blue fluorescent staining).