Table 2.
Isoform specific monoclonal and polyclonal antibodies employed in this study.
| Antibody | Isoform | Working Dilution | Source | Reference |
|---|---|---|---|---|
| mAb 9A7 (pan α monoclonal) | α isoforms | 1:100 | M. Takahashi | [47] |
| α5 § (monoclonal) | α isoforms | Neat Supernatant | D.M. Fambrough and DSHB * | [48] |
| α6F (monoclonal) | α1 | Neat Supernatant | D.M. Fambrough and DSHB * | [49] |
| α620 (polyclonal) | α1 | 1:100–1:200 | M. Caplan | [50,51] |
| McB2 (monoclonal) | α2 | Neat Supernatant | K.J. Sweadner | [52] |
| McBX3 (monoclonal) | α3 | Neat Supernatant | K.J. Sweadner | [30,46] |
| XVIF9G10 (monoclonal) | α3 | Neat Supernatant | K.J. Sweadner | [30,46] |
| SpETb1 (polyclonal) | β1 | 1:200–1:400 | P. Martín-Vasallo | [53] |
| SpETb2 (polyclonal) | β2 | 1:200–1:400 | P. Martín-Vasallo | [53] |
*
The α5 and α6F monoclonal antibodies were developed by D. Fambrough were obtained from the Developmental Studies Hybridoma Bank (DSHB [54]) developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biological Sciences, Iowa City, IA 52242, USA.
§
The α5 monoclonal broadly recognizes Na+, K+-ATPase α subunits of avian, mammalian and insect species. There are 4 known α isoforms and the designation “α5” does not indicate the existence of a fifth Na+, K+-ATPase α isoform.