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. Author manuscript; available in PMC: 2012 Oct 4.
Published in final edited form as: Cell Metab. 2012 Apr 4;15(4):439–450. doi: 10.1016/j.cmet.2012.02.014

Fig. 3.

Fig. 3

mir-71 functions in the AB lineage to regulate the lifespan of germline-deficient animals. (A) A Pmir-71::gfp reporter was strongly expressed in the intestine, body wall muscles and neurons during adulthood. (B) A cell-lineage diagram indicating tissues produced by the early blastomeres of C. elegans. (C–E) mir-71 functions primarily in AB-derived tissue(s) to mediate the effects of germline on lifespan. Germline removal had a minor effect on the lifespan of mir-71 mutants (p<0.0001; 7% mean lifespan extension), whereas it robustly extended the lifespan of animals that carried the mir-71-expressing array presumably in all tissues (p<0.0001; 60% mean lifespan extension). Whereas mir-71 E(−) mosaics fully responded to germline removal (p<0.0001; 60% mean lifespan extension), the lifespan of mir-71 AB(−) mosaics was only modestly extended by germ cell loss (p<0.0001; 23% mean lifespan extension). Mean lifespan values and statistical analyses of lifespan assays are shown in Supplementary Table 2.