Fig. 4.

Expression of mir-71 in neurons alone was sufficient to promote germline-mediated longevity. (A–D) Driving mir-71 expression either ubiquitously (using the rpl-28 promoter) or in the nervous system of mir-71 mutants (using the pan-neuronal unc-119 promoter) resulted in strong rescue (p<0.0001; 40–45% mean lifespan extension). On the other hand, hypodermal-specific expression of mir-71 (using the dpy-7 promoter) failed to rescue the lifespan defect of mir-71; glp-1 mutants (p<0.0001; 5–10% mean lifespan extension). All experiments were repeated at least once with similar effects. Mean lifespan values and statistical analyses of lifespan assays are shown in Supplementary Table 2.