Figure 1.

RhoB is dispensable for arrest response of primary cells after DNA damage. (A) PCR genotyping. Genotypes were determined by multiplex PCR for rhoB or neo transgene sequences as described (14). Products were fractionated on agarose gels, stained with ethidium bromide, and photographed. (B) Flow cytometry. Primary MEFs (passage 3) were subjected to treatment with 1,000 ng/ml doxorubicin (Dox) or a 20-Gy dose of gamma irradiation and processed for flow cytometry 24 h or 36 h later, respectively. MEFs prepared from different embryos responded similarly.