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. 2001 May 22;98(11):6198–6203. doi: 10.1073/pnas.101579798

Figure 1.

Figure 1

Activation of JNK cascade inhibits Smad2-dependent transcription. (A) Mv1Lu cells were transfected with ARE-Lux alone or with FAST1 in the presence or absence of constitutively activated mutants of MEKK1 (MEKK1.EE) and MKK4 (MKK4.ED). (B) HepG2 cells were transfected with gsc-Lux and FAST2 in the presence or absence of MEKK1.EE and MKK4.ED. (C) Mv1Lu cells were transfected with ARE-Lux alone or with FAST1 in the presence of absence of dominant-negative mutants of MEKK1 (MEKK1.K432A) and MKK4 (MKK4.Ala). In all cases, cells were treated with (filled bars) or without (open bars) TGF-β for 16 h before lysis and then assayed for luciferase activity. Luciferase activity was normalized to β-galactosidase activity and was expressed as mean ± SD of triplicates from a representative experiment performed at least three times.