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. 2001 May 22;98(11):6198–6203. doi: 10.1073/pnas.101579798

Figure 2.

Figure 2

c-Jun inhibits Smad2 transcriptional activity. (A) COS-7 cells were transfected with Myc-FAST1 and Flag-Smad2 in the presence or absence of MKK4.ED and wild-type (HA-TβRI), or constitutively activated (HA-TβRI.act) TGF-β type I receptor. Cell lysates were subjected to immunoprecipitation with anti-Myc antibody and then immunoblotted with anti-Flag antibody. The expression of transfected DNA was determined by immunoblotting whole cell extracts with anti-Myc or anti-Flag antibodies. (B) HepG2 cells were cotransfected with ARE-Lux, together with FAST1 and HA-c-Jun or HA-c-JunbZip, and cell lysates were assayed for luciferase activity. Cells were treated with (filled bars) or without (open bars) TGF-β for 16 h before lysis and then assayed for luciferase activity (Upper). Expression of HA-c-Jun or HA-c-JunbZip was assessed by Western blotting of cell lysates with anti-HA antibody (Lower).