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. 2001 May 22;98(11):6198–6203. doi: 10.1073/pnas.101579798

Figure 3.

Figure 3

c-Jun stabilizes the Smad2/TGIF complex. (A) COS-7 cells were transfected with Flag-p300(1892–2441) and Myc-Smad2 in the presence or absence of HA-c-Jun and HA-TβRI or HA-TβRI.act. Association of p300(1892–2441) with Smad2 was analyzed by blotting the Flag immunoprecipitates with the anti-Myc antibody. (B) COS-7 cells were transfected with various combinations of Flag-TGIF, Myc-Smad2, HA-c-Jun, and HA-TβRI or HA-TβRI.act as indicated. Flag immunoprecipitates were subjected to immunoblotting with anti-Myc or anti-HA antibodies.