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. 2001 May 22;98(11):6198–6203. doi: 10.1073/pnas.101579798

Figure 5.

Figure 5

Expression of the mutant c-JunbZip inhibits TGIF-mediated repression of Smad2 transcriptional activity. (A) HA-c-Jun or HA-c-JunbZip was cotransfected in COS-7 cells with Flag-TGIF and with HA-TβRI or HA-TβRI.act. Cell lysates were subjected to anti-Flag immunoprecipitation and then immunoblotted with anti-HA antibody. (B) Cell lysates from transiently transfected COS-7 cells were subjected to immunoprecipitation with anti-Myc antibody and then immunoblotted with anti-Flag antibody. (C) HepG2 cells were cotransfected with the indicated combination of ARE-Lux, FAST1, TGIF, HA-c-Jun, and HA-c-JunbZip. Cells were treated with (filled bars) or without (open bars) TGF-β for 16 h before lysis and then assayed for luciferase activity.