Figure 5.

Cytokine secretion profile of phPLP139-151, 175-194, and 257-276-primed LNC derived from immunized DRB1*1501- or DQB1*0602-Tg mice. HLA-DRB1*1501- and DQB1*0602-Tg mice were immunized by s.c. injection of 150 μg phPLP139-151, phPLP175-194 or phPLP257-276 (as control) in CFA. Ten days later, draining LNC (pooled from 3 mice) were cultured for 48 h without or with the immunizing peptide, respectively, and supernatants were analyzed for the secretion of indicated cytokines. Values presented (pg/ml) are after background (without antigen) cytokines were subtracted. The results represent three experiments done in triplicates, and are the mean cytokine concentration ± SE.