Table 1.
Survival of inbred ES cell clones
ES cell line | Genotype | Total active PN | Blastocyst stage-ET (% PN) | Pups alive at term (% ET) | Pups surviving to adulthood |
---|---|---|---|---|---|
J1 | 129/Sv | 352 | 68 (19) | 6 (9) | 0 |
V18.6 | 129/Sv | 178 | 40 (22) | 7 (18) | 0 |
V26.2 | C57BL/6 | 164 | 40 (24) | 2 (5) | 0 |
V39.7 | BALB/c | 123 | 34 (28) | 0 (0) | 0 |
Total | — | 817 | 182 (22) | 15 (8) | 0 |
Development of inbred ES cell clones. Total active PN refers to the number and percent of reconstructed oocytes with observable PN. Blastocyst stage-ET refers to the number and percent of embryos with PN that developed to the blastocyst stage and subsequently were transferred to pseudo-pregnant recipient females.