Figure 1.

Multi-channel microscopy procedure for imaging dynamic samples. a–b All channels are captured simultaneously on microscopes equipped for parallel imaging a Sample undergoing a periodic motion pattern imaged in three channels b Composite image of all channels. c–d Channels are captured sequentially on microscopes equipped with a single detector/camera c Sample undergoing a periodic motion pattern imaged alternatively in three channels d inaccurate composite image due to asynchronous imaging of the channels. e–f Channels captured sequentially at high frame rate over one ore more occurences of the motion pattern e Each channel is captured at full camera frame rate with short delays in between channels f random triggering leads to sequences that are temporally unregistered. g–h Multi-modal registration allows for high-speed multi-modal imaging g Temporal registration procedure of the sequences acquired in panel e. f Aligned images yield composite images at full frame rate.