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. 2012 May 4;7(5):e35943. doi: 10.1371/journal.pone.0035943

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Westerman et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Strategy for targeting the CAG promotor-LoxP-PGK-Neo-3xtranscriptionstop-LoxP-Bmi1 cDNA-IRES-Hyg/eGFP cassette into the ROSA26 locus. (B) Southern blot showing germ line transmission of two out of 7 mice. (C) Western blot showing Adenoviral-Cre mediated expression of Bmi1 in cultured mammary epithelial cells (MECS) of a Bmi1^LSL transgene mouse compared to a wild type (WT) mouse. Bmi1 is detected in MECS that received 50 or 100 virus particles per cell. MOI, multiplicity of infection. (D) Immunohistochemistry showing nuclear transgenic Bmi1 expression of hepatocytes of adult mice after adenocre (Ad5Cre) mediated activation using intravenous injection of 10⁹ infectious particles. (E) Immunohistochemistry showing Bmi1 expression in the small intestine and liver of Actin-Cre (Acre) or Acre;Bmi1 mice of embryonic day 18.5 mice.