Figure 2. Constitutive transgenic expression of Bmi1^LSL is neonatally lethal.

(A) Histogram showing that constitutive expressing Acre;Bmi1^LSL mice have the same growth kinetics as wild type control mice in utero between embryonic day E12.5 and E18.5. (B) Histogram showing that Acre;Bmi1^LSL transgene mice have less nucleated red blood cells in their lungs compared to Acre control mice at embryonic day E18.5. Nucleated red blood cells are visualized using PTAH staining resulting in blue nuclei, as shown in the lower panels. *p = <0.05, NS: not significant.