Figure 6. Effect of cross-linking of CTLA-4 and LIR-1 on T cell responses against T. cruzi-antigens.

IFN-γ ELISPOT responses of PBMCs from T. cruzi-infected subjects stimulated with T. cruzi lysate or media alone were measured in the presence of a plate bound isotype control, anti-CTLA-4 or anti-LIR-1 antibodies. The data represent the mean spot number/10⁶ PBMCs for individual subjects with positive IFN-γ ELISPOT responses prior to cross-linking assays. (*) Indicates significant differences in T. cruzi-specific IFN-γ ELISPOT responses (SFCs in media subtracted) between previous and post cross-linking assays, as described in Material and Methods. The data represent the mean SFCs number/1×10⁶ PBMCs. ^(a) LIR-1 cross-linking was not performed. The clinical status of each subject is indicated between brackets.