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. 2012 May 4;7(5):e35966. doi: 10.1371/journal.pone.0035966

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Laucella et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Double immunofluorescence staining with CD3 and CTLA-4 antibodies was performed as described in Material and Methods. From total CD3-expressing T cells present in the inflammatory infiltrate (A) a small proportion showed CTLA-4 expression (B). Nuclei staining with DAPI. The arrowheads point the nuclei of CTLA4⁺ cells (C). Composite of figures A, B and C showing the double stained cells (arrowheads) and a CD3⁺CTLA-4⁻ single stained cell (large arrow)(D). Original Magnification 400×.