Figure 1. Translatomes of NIH3T3 and Jurkat cells based on polysome profiling.
A) The quality of polysome preparation was verified by electrophoretic analysis of RNA content in each fraction. The effect of thapsigargin treatment (stress) for the indicated hours on polysome distribution in Jurkat cells is also shown. A comparable result was obtained in NIH3T3 cells (Figure S1). Ribosomal 18 S and 28 S bands are shown. B) and C) Density distribution of translation efficiencies of 10,670 coding mRNAs from Jurkat and 8,459 mRNAs from NIH3T3 under optimal growth conditions. Those mRNA equally distributed among FM and P fractions showed a log2P/FM = 0 (marked by a dotted red line). The median values for Jurkat and NIH3T3 cells were 1.07 and 0.45, respectively. The translation efficiencies of some representative mRNA are shown (ACTB, HSPA5, SNRPB, RPL4, ATF4 and FTH1). D) qPCR validation of translation efficiencies for some of the above mentioned mRNA. Data are the mean ±SD from two independent qPCR reactions in Jurkat and NIH3T3 cells.