Table 6. Summary of mitochondrial markers significantly associated 1 with growth traits and their annotations1.
SNP | Physical position (bp)2 | MAF3 | Annotation4 | Location/amino acid change |
mtSNP1 | 4116 | 0.49 | NADH dehydrogenase subunit 1 | ORF/SYN |
mtSNP2 | 4323 | 0.49 | NADH dehydrogenase subunit 1 | ORF/SYN |
mtSNP3 | 4647 | 0.48 | NADH dehydrogenase subunit 1 | ORF/SYN |
mtSNP4 | 5212 | 0.48 | NADH dehydrogenase subunit 2 | ORF/SYN |
mtSNP5 | 5275 | 0.48 | NADH dehydrogenase subunit 2 | ORF/SYN |
mtSNP6 | 5530 | 0.48 | NADH dehydrogenase subunit 2 | ORF/SYN |
mtSNP7 | 5740 | 0.49 | NADH dehydrogenase subunit 2 | ORF/SYN |
mtSNP16 | 12423 | 0.47 | NADH dehydrogenase subunit 4 | ORF/V→ M |
mtSNP17 | 13231 | 0.49 | NADH dehydrogenase subunit 5 | ORF/SYN |
mtSNP18 | 13795 | 0.48 | NADH dehydrogenase subunit 5 | ORF/SYN |
mtSNP19 | 14077 | 0.48 | NADH dehydrogenase subunit 5 | ORF/SYN |
mtSNP20 | 14626 | 0.49 | NADH dehydrogenase subunit 5 | ORF/SYN |
mtSNP21 | 15591 | 0.23 | Cytochrome b | ORF/SYN |
mtSNP22 | 15822 | 0.49 | Cytochrome b | ORF/SYN |
mtSNP23 | 16305 | 0.49 | Cytochrome b | ORF/SYN |
mtSNP24 | 16317 | 0.48 | Cytochrome b | ORF/SYN |
mtSNP8 | 7052 | 0.23 | Cytochrome c oxidase subunit 1 | ORF/SYN |
mtSNP9 | 7193 | 0.50 | Cytochrome c oxidase subunit 1 | ORF/SYN |
mtSNP10 | 8774 | 0.48 | Cytochrome c oxidase subunit 2 | ORF/SYN |
mtSNP11 | 8804 | 0.48 | Cytochrome c oxidase subunit 2 | ORF/SYN |
mtSNP14 | 9410 | 0.48 | ATPase 6 | ORF/SYN |
mtSNP15 | 9656 | 0.49 | ATPase 6 | ORF/SYN |
mtSNP12 | 9084 | 0.48 | ATPase 8 | ORF/SYN |
mtSNP13 | 9087 | 0.49 | ATPase 8 | ORF/SYN |
Body weight was recorded on each animal at approximately 7 (Weight2), 9 (Weight3) and 12 (Weight4) months post-hatching. Population-based association analysis was performed with program PLINK version 1.07 [22]. From 40 full-sib families each with ∼17 progeny, a sibling was randomly sampled from each family to generate a population-based sample of n = 40 unrelated individuals; we repeated the random sampling to develop three sets of unrelated individuals.
Markers were positioned on mitochondrial genome by BLASTing sequences flanking markers against a rainbow trout mitochondrial reference sequence [33].
SNPs minor allele frequency (MAF). 4SNP annotation; gene name and SNP location (ORF/5′UTR/3′UTR), SYN = Synonymous.