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. 2012 May 4;7(5):e36282. doi: 10.1371/journal.pone.0036282

Figure 4.

Figure 4

(a) Intrachain entanglement (“self-entanglement”) and (b) interchain entanglement measured by the mean average crossing number mACN as a function of chain rigidity Inline graphic. Tethering the SC polymer's ends to the borders of the confining cavity induces fewer chain overcrossings than the “null model” consisting of free semiflexible polymers in confinement, which suggests that the interplay between tethering and confinement might help to prevent an excess of chain overcrossings. However, semiflexibility induces a trade-off in both polymer systems between the amount of intrachain- and interchain-entanglement which has to be balanced with respect to interlock resolution. Notably, we find a surprisingly low amount of both types of chain overcrossings for the 4Pi microscopy dataset, mACNInline graphic and mACNInline graphic, which cannot be explained within our SC polymer model.