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. 2012 May 4;7(5):e36372. doi: 10.1371/journal.pone.0036372

Figure 8. FoxM1 mRNA level increases after Cdc7 depletion in HeLa and p53-negative HCT116.

Figure 8

(A) HeLa cells were treated with indicated siRNAs for 24 hrs. FoxM1 (left), Plk1 (middle) and CyclinB1 (right) mRNA levels are presented. (B) Western analysis of the whole cell extracts of HeLa cells treated with indicated siRNAs for 48 hrs. A phosgel was used for the detection of MK2. Other proteins were separated on a 4–12% gradient gel. (C) The FoxM1 mRNA levels of HCT116 (p53-positive and -negative) cells treated with control or Cdc7 siRNA for 24 hrs. In A and C, mRNA levels were quantified by real time-PCR and the relative values normalized by the level of GAPDH mRNA are presented. (D) HeLa cells treated with indicated siRNAs for 48 hrs were fixed with 4% paraformaldehyde for 10 min and stained with anti-CyclinB1 antibody. Fractions of the cells showing nuclear localization of CyclinB1 are shown. Cdc7-D siRNA was used in these experiments.