Figure 4. Alternative approaches to Ni(II)-dependent affinity tag cleavage in protein purification.
The tag can be removed in solution (left) or when immobilized at the affinity column (right).
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.