Table 1. The temperature dependence of the 1st order rate constant and reaction half-times for SPI2-SRHWAP-His6 hydrolysis in solution, at H 8.2, determined by HPLC.
T [°C] | k ± S.D. (s−1×10−5) | t1/2 (h) |
50 | 4.10±0.63 | 5 |
45 | 2.05±0.11 | 9 |
40 | 0.76±0.08 | 25 |
37 | 0.22±0.07 | 86 |
28 | 0.09±0.005 | 206 |