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. 2012 May 4;7(5):e36519. doi: 10.1371/journal.pone.0036519

Figure 5. Kinetic mechanism of l-iLDH from P. stutzeri SDM.

Figure 5

Purified l-iLDH (0.1 µg) was incubated in a reaction mixture containing 50 mM Tris-HCl (pH 7.5) at 30°C. (a) The reaction was started with different l-lactate concentrations at variable MTT concentrations. ▪, 0.2 mM MTT; •, 0.25 mM MTT, ▴, 0.35 mM MTT; ▾, 0.75 mM MTT; ⧫, 0.1 mM MTT. (b) The reaction was started with different MTT concentrations at variable l-lactate concentrations. ▪, 0.02 mM l-lactate; •, 0.0325 mM l-lactate; ▴, 0.05 mM l-lactate; ▾, 0.0825 mM l-lactate; ◂, 0.125 mM l-lactate; ▸, 0.1675 mM l-lactate; ⧫, 0.25 mM l-lactate; <$>\scale 80%\raster="rg1"<$>, 0.375 mM l-lactate.