Table 2. The strains used in this work.
Strain | Characteristics a | Source or reference |
P. stutzeri SDM | Undomesticated wild strain capable of l-Lactate utilizing | [13] |
P. stutzeri SDM-dlldD | Kmr; P. stutzeri SDM mutant obtained by disruption of the lldD gene | This study |
P. stutzeri SDM-dlldD(pBSP II SKlldD) | Kmr; Apr; P. stutzeri SDM-dlldD harboring the plasmids pBSP II SKlldD | This study |
E. coli DH5α | F− SupE44, Δ(argF lacZya)U169 ϕ80lacZΔM15 hsdR17(rk −,mk +) recA1 endA1 gyrA96 thi-1 relA1 λ− | Invitrogen |
E. coli DH10B | F− endA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 Φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ− | Invitrogen |
E. coli HB101 | F− mcrB mrr hsdS20(rB − mB −) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ− | Invitrogen |
E. coli C43 (DE3) | Mutant of E. coli BL21 (DE3) for membrane-bound protein expression | [39] |
Kmr and Apr indicate resistance to kanamycin and ampicillin, respectively.