Skip to main content
. 2012 May 4;7(5):e36595. doi: 10.1371/journal.pone.0036595

Figure 2. Leishmania isolate infectivity and PS exposure.

Figure 2

Peritoneal macrophages derived from F1 mice were infected with different isolates obtained from patients with LCL (BA69, BA73, BA 125, and M2269) (□) and DCL (BA276, BA336, and BA700) (▪). After 5, 24 and 72 h of infection, cells were fixed and stained. The percentage of infected macrophages (A) and infectivity index (B) were defined under a microscope. Parasite burden was measured by quantitative PCR at 24 and 72 h (C). Boxes represent median values and interquartile interval from different isolates mentioned above. The correlations between PS exposure at 24 h with percentage of infected macrophages and infectivity index in 72 h are showed in (D) and (E), respectively. The four lower points in X axis (PS exposure) represents LCL isolates while the three higher points are from DCL isolates. One representative experiment of at least three independent repeats is shown. Kruskal-Wallis was used with Dunn's Multiple Comparison post-test. Spearman test was used to verify the correlations. The r values are plotted in each graph.