Figure 3. Antibacterial activity in Rhodnius prolixus 5^th-instar nymphs challenged by Trypanosoma cruzi Dm28c clone.

Anterior midgut samples collected nine days after feeding and infection. (A) Inhibition zone (ZI) assay incubated for 24 h at 37°C. (B) Turbidometric (TB) assay incubated for 11 h with readings at each hour. (C) Turbidometric (TB) assay after 4 h of incubation of uninfected or infected control insects. (D) Turbidometric assay after 11 h of incubation of insects treated with antibiotic alone or with antibiotic and then infected. Treatments: C – control insects fed on blood alone; CC – parasite infected insects; A – insects treated with antibiotic; AC- insects treated with antibiotic and then infected with parasites. Each bar represents mean ± SEM of four experiments, n = 6–10 insects for each determination. Means were analyzed by using t Test, Mann Whitney test and 2 way ANOVA.