Table 1. Electrophysiological properties of ASC and of the derived in vitro-differentiated adipocytes.
UNITS | S-ASC | V-ASC | S-ADIPO | V-ADIPO | |
RMP | mV | −50.1±5.3 | −45.6±4.3* | −52.4±5.6 | −43.1±4.8** |
Cm | pF | 12.0±1.2 | 19.1±1.6** | 27.3±2.2§§ | 16.2±1.6**§§ |
Gm | nS | 31.2±2.8 | 45.6±4.1** | 24.5±2.2 | 30.4±2.8**§§ |
Gm/Cm | nS/pF | 2.6±0.2 | 2.4±0.2 | 0.9±0.1§ | 1.9±0.2**§ |
Vth (IKs) | mV | −24.3±2.7 | −12.4±2.6*** | −12.3± 2.9§§§ | −10.3±2.7 |
Vth (IBK) | mV | −22.1±2.9 | −12.2±2.1*** | −32.8± 3.0§§§ | −32.6±2.5§§§ |
Vth (IKir) | mV | −72.1±7.1 | −69.2±8.1 | −72.4±6.8 | −72.5±7.4 |
τ (IKs) | ms | 160.5±15.1 | 220.5± 19.2*** | 180.5± 14.6§ | 200.8± 18.4***§ |
τ (IBK) | ms | 78.3±7.6 | 36.4±6.3*** | 40.3±5.6§§ | 50.2±6.3*§§ |
IKs/Cm | pA/pF | 60.7±5.5 | 14.2±1.3*** | 4.2±0.4§§§ | 2.5±0.2**§§§ |
IBK/Cm | pA/pF | 33.6±3.0 | 17.9±1.6** | 8.0±0.7§§§ | 11.3±1.0**§§ |
IKir/Cm | pA/pF | −10.2±1.3 | −9.8±1.0 | −101± 10.2§§§ | −59± 7.1***§§§ |
Electrophysiological analysis was performed on ASC and their corresponding in vitro-differentiated adipocytes in patch clamp condition. RMP: resting membrane potential. Cm: membrane capacitance. Gm and Gm/Cm: total and specific membrane conductance, respectively. Vth: voltage threshold of activation of the three types of K+ currents investigated (IBK, IKs and IKir). K+ current activation time constant (τ) for IBK and IKs and current density IBK/Cm and IKs/Cm values have been obtained at 50 mV and IKir/Cm at −110 mV. Mean ±SE values are reported. Student's t test was applied to compare paired samples: **,***P<0.01 and 0.001 V- versus S-ASC and V- versus S-ADIPO; §, §§, §§§ P<0.05, 0.01, 0.001 S- or V-ADIPO versus the corresponding ASC. Total number of analyzed cells (n) in populations obtained from 5 different subjects were: S-ASC (n = 84), V-ASC (n = 82), S-ADIPO (n = 54), V-ADIPO (n = 52); for IKir: S-ASC (n = 17), V-ASC (n = 18), S-ADIPO (n = 28), V-ADIPO (n = 24). mV: milli Volt; pF: pico Faraday; nS: nano Siemens; ms: milli seconds; pA: pico Ampere.