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. 2012 May 4;7(5):e36569. doi: 10.1371/journal.pone.0036569

Table 1. Electrophysiological properties of ASC and of the derived in vitro-differentiated adipocytes.

UNITS S-ASC V-ASC S-ADIPO V-ADIPO
RMP mV −50.1±5.3 −45.6±4.3* −52.4±5.6 −43.1±4.8**
Cm pF 12.0±1.2 19.1±1.6** 27.3±2.2§§ 16.2±1.6**§§
Gm nS 31.2±2.8 45.6±4.1** 24.5±2.2 30.4±2.8**§§
Gm/Cm nS/pF 2.6±0.2 2.4±0.2 0.9±0.1§ 1.9±0.2**§
Vth (IKs) mV −24.3±2.7 −12.4±2.6*** −12.3± 2.9§§§ −10.3±2.7
Vth (IBK) mV −22.1±2.9 −12.2±2.1*** −32.8± 3.0§§§ −32.6±2.5§§§
Vth (IKir) mV −72.1±7.1 −69.2±8.1 −72.4±6.8 −72.5±7.4
τ (IKs) ms 160.5±15.1 220.5± 19.2*** 180.5± 14.6§ 200.8± 18.4***§
τ (IBK) ms 78.3±7.6 36.4±6.3*** 40.3±5.6§§ 50.2±6.3*§§
IKs/Cm pA/pF 60.7±5.5 14.2±1.3*** 4.2±0.4§§§ 2.5±0.2**§§§
IBK/Cm pA/pF 33.6±3.0 17.9±1.6** 8.0±0.7§§§ 11.3±1.0**§§
IKir/Cm pA/pF −10.2±1.3 −9.8±1.0 −101± 10.2§§§ −59± 7.1***§§§

Electrophysiological analysis was performed on ASC and their corresponding in vitro-differentiated adipocytes in patch clamp condition. RMP: resting membrane potential. Cm: membrane capacitance. Gm and Gm/Cm: total and specific membrane conductance, respectively. Vth: voltage threshold of activation of the three types of K+ currents investigated (IBK, IKs and IKir). K+ current activation time constant (τ) for IBK and IKs and current density IBK/Cm and IKs/Cm values have been obtained at 50 mV and IKir/Cm at −110 mV. Mean ±SE values are reported. Student's t test was applied to compare paired samples: **,***P<0.01 and 0.001 V- versus S-ASC and V- versus S-ADIPO; §, §§, §§§ P<0.05, 0.01, 0.001 S- or V-ADIPO versus the corresponding ASC. Total number of analyzed cells (n) in populations obtained from 5 different subjects were: S-ASC (n = 84), V-ASC (n = 82), S-ADIPO (n = 54), V-ADIPO (n = 52); for IKir: S-ASC (n = 17), V-ASC (n = 18), S-ADIPO (n = 28), V-ADIPO (n = 24). mV: milli Volt; pF: pico Faraday; nS: nano Siemens; ms: milli seconds; pA: pico Ampere.