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. 2012 May 4;7(5):e36764. doi: 10.1371/journal.pone.0036764

Figure 7. PKM2 over-expression abolished the effects of resveratrol.

Figure 7

Over-expression of PKM2 using myc tagged pCDNA vector (see text) was confirmed using anti myc and anti PKM2 antibodies (A). Increased cellular proliferation in PKM2 transfected cells, compared to mock transfected cells, in continuous presence of inhibitory concentrations of resveratrol (B). Glucose uptake (C) and lactate production (D) was substantially enhanced in PKM2 transfected cells indicating that PKM2 over-expression abrogates effects of resveratrol on cancer metabolism and cellular proliferation. These results further validate PKM2 as critical target of resveratrol. All experiments were repeated 3 times and data expressed as mean ± SE. *p<0.05. PKM2 – indicates mock transfected cells whereas PKM2+indicates PKM2 transfected cells.