Fig. 3.

ema is dispensable for autophagosomal fusion with endosomes and lysosomes. (A) Autophagosomal fusion with lysosomes. Representative single confocal sections show part of a starved Drosophila fat body cell that expresses both the lysosomal marker Lamp1-GFP (green) and the autophagosomal marker mCherry-Atg8a (red). Arrowheads indicate autolysosomes (fusion products of autophagosome and lysosome) that contain both Lamp1-GFP and mCherry-Atg8a. Wild type = CG-Gal4/+,UAS-Lamp1-GFP/+,UAS-mCherry-Atg8a/+; Ema = CG-Gal4/+,UAS-Lamp1-GFP/+,UAS-mCherry-Atg8a/+, ema¹. (B) Autophagosomal fusion with endosomes. Representative single confocal sections of fat body cells expressing the autophagosomal marker mCherry-Atg8a (green) and labeled with the endocytic tracer avidin-Cy3 (red). Arrowheads indicate an amphisome (fusion product of autophagosome and endosome) that contains both GFP-Atg8a and avidin-Cy3. White arrows indicate autophagosomes that have not fused with endosomes. Yellow arrows indicate an endosome that has not fused with autophagosomes. Wild type = da-Gal4/+,UAS-GFP-Atg8a/+; Ema = da-Gal4/+,UAS-GFP-Atg8a/+,ema¹.