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. 2012 Apr 13;109(18):6963–6968. doi: 10.1073/pnas.1118754109

Fig. 5.

Fig. 5.

Effect of knockdown of β-catenin on the malignant phenotype of primary glioma cell lines. (A) Formation of tumor spheres (Upper) and analysis of the cell cycle (Lower) of primary glioma cells before and after knockdown of β-catenin with two different siRNA constructs. (Magnification: 40×.) (B) (Left) Assay of the formation of spheres in tumors. (Right) Diameter of the spheres before and after knockdown of β-catenin with two different siRNA constructs. (C) Immunofluorescence staining of primary glioma cells for β-catenin (green) and c-Myc (red) before and after siRNA knockdown of β-catenin. (Scale bar: 10 μm.) (D) Immunofluorescence staining of primary glioma cells for SOX2 (green) and Nestin (red). (Scale bar: 10 μm.)