Table 1.
Activities of hydrocarbon formation by cofactor-deficient MoFe proteins in Eu(II)-DTPA–driven reactions
| Activities |
|||
| Products | ΔnifB MoFe protein | ΔnifBΔnifZ MoFe protein | ΔnifH MoFe protein |
| From CN− | |||
| CH4 | 91 ± 14 | 468 ± 49 | 1,280 ± 204 |
| C2H4 | 85 ± 9 | 515 ± 51 | 1,230 ± 114 |
| C2H6 | 18 ± 2 | 117 ± 10 | 250 ± 20 |
| C3H6 | 20 ± 3 | 182 ± 13 | 475 ± 51 |
| C3H8 | 2.7 ± 0.3 | 36 ± 3 | 72 ± 9 |
| C4H8 | ND | 72 ± 8 | 160 ± 19 |
| C4H10 | ND | 7 ± 1 | 24 ± 2 |
| C5H10 | ND | 2.1 ± 0.3 | 67 ± 9 |
| C5H12 | ND | ND | 13 ± 1 |
| NH3 | 1,193 ± 114 | 4,648 ± 410 | 10,180 ± 1131 |
| From CO | |||
| CH4 | 6 ± 1 | 25 ± 3 | 83 ± 11 |
| C2H4 | 2.7 ± 0.3 | 11 ± 1 | 29 ± 4 |
| C2H6 | 0.9 ± 0.1 | 4 ± 4 | 14 ± 2 |
| C3H6 | ND | 1.4 ± 0.2 | 5 ± 1 |
| C3H8 | ND | 0.5 ± 0.1 | 2.7 ± 0.3 |
Activities are expressed in nanomole product/micromole protein per hour. See Materials and Methods for assay conditions. ND, not detectable.