Fig. 3.

STIM2 does not contribute to cysLT1 receptor-evoked Ca²⁺ oscillations over 600 s. (A) Western blot shows significant reduction in STIM2 expression after siRNA transfection. (Lower) Depicts aggregate data from three independent results. (B) Confocal microscopy images comparing protein expression in mock-transfected cells and in those in which STIM2 had been knocked down. (C) Histogram depicts aggregate data from three independent experiments (each bar is the average from >18 cells). (D) The trace represents a typical oscillatory Ca²⁺ signal to LTC₄ in a control cell. (E) The recording depicts a typical oscillatory Ca²⁺ signal to LTC₄ in which STIM2 had been knocked down. (F) Aggregate data from several cells are compared (each point represents 18–46 cells).