Fig. 5.

FCεRI receptor activation evokes Ca²⁺ signals and gene expression through STIM1 and STIM2. (A) Typical Ca²⁺ signal to IgE in a control cell. (B) Ca²⁺ response to IgE in the absence of external Ca²⁺. (C) Ca²⁺ signal to IgE after knockdown of STIM1. (D) Ca²⁺ signal to IgE after knockdown of STIM2. (E) NFAT-dependent GFP expession after stimulation with IgE in mock-transfected cells (Top) and after knockdown of STIM1 (Middle) or STIM2 (Bottom). (F) Aggregate data from several experiments are compared. *P < 0.05 and **P < 0.01.