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PKM2 maintains mTORC1 activity upon serine depletion. (A) M2 cells, but not M1 cells, maintain mTORC1 activity upon serine deprivation. Flag-M1/M2 (Left) or HA-M1/M2 (Right) cells were incubated with/without serine for 16 h, and mTOR activities were measured by immunoblotting for p-S6K and p-S6. Data are representative of three independent experiments. (B) Leucine withdrawal reduces mTORC1 activity in both M1 and M2 cells. Flag-M1/M2 cells were incubated with/without leucine for 16 h.
