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. 2012 May 4;7(5):e36655. doi: 10.1371/journal.pone.0036655

Figure 8. 0-mpf inhibition of GSK-3 activity randomized the parallel microtubule arrays at the vegetal pole and the biased migration of Wnt8a transcripts.

Figure 8

Microtubule staining with an anti-β-tubulin antibody to visualize the microtubule arrays formed at around 20 mpf at the vegetal pole. (A) Parallel microtubule arrays detected in NaCl treated embryos. (B) Randomized aligned microtubule arrays detected in 0-mpf lithium treated embryos. (C) Similar phenomenon detected in 0-mpf GSK-3 inhibitor treated embryos. (D–F) 0-mpf lithium and GSK-3 inhibitor IX treatments disrupted the polarized distribution of Wnt8a mRNA observed at 4-cell stage. (D) A 0-mpf NaCl treated wild-type embryo, (E) A 0-mpf lithium treated wild-type embryo, (F) A 0-mpf GSK-3 inhibitor IX treated wild-type embryo (G) Wnt8a mRNA restricted to the animal pole region of the 0-mpf NaCl treated 4-cell-stage tkk mutant embryos. (H I) Exposure of GSK-3 inhibitors failed to alter the distribution of Wnt8a mRNA in 4-cell-stage tkk mutant embryos.