Table 1.
Localization of developmental proteins in podJ mutants
| Strain | % Flagellar pole only* | % Stalked pole only* | % Bipolar* | % No signal detected | Total cells counted |
|---|---|---|---|---|---|
| CpaE: | |||||
| Wild-type | 61 ± 11 | 0 ± 0 | 1 ± 1 | 38 ± 11 | 1333 |
| ΔpodJ | 3 ± 3 | 26 ± 6 | 4 ± 2 | 67 ± 8 | 1228 |
| podJΔ589-639 | 5 ± 2 | 17 ± 11 | 4 ± 1 | 73 ± 14 | 1502 |
| podJΔ921-974 | 27 ± 6 | 7 ± 1 | 12 ± 4 | 55 ± 6 | 1306 |
| podJΔ589-639 Δ921-974 | 4 ± 1 | 13 ± 7 | 3 ± 2 | 81 ± 8 | 1333 |
| PleC: | |||||
| Wild-type | 44 ± 5 | 0 ± 0 | 0 ± 0 | 56 ± 5 | 2357 |
| ΔpodJ | 3 ± 1 | 9 ± 2 | 2 ± 1 | 86 ± 4 | 1203 |
| podJΔ589-639 | 16 ± 6 | 2 ± 2 | 1 ± 1 | 81 ± 9 | 900 |
| podJΔ921-974 | 36 ± 6 | 0 ± 0 | 1 ± 0 | 63 ± 6 | 853 |
| podJΔ589-639 Δ921-974 | 3 ± 1 | 5 ± 1 | 1 ± 1 | 91 ± 2 | 799 |
| DivL: | |||||
| Wild-type | 40 ± 9 | 3 ± 1 | 10 ± 6 | 47 ± 16 | 912 |
| ΔpodJ | 3 ± 1 | 30 ± 3 | 24 ± 4 | 43 ± 2 | 894 |
| podJΔ589-639 | 14 ± 0 | 13 ± 0 | 28 ± 0 | 45 ± 1 | 822 |
| podJΔ921-974 | 17 ± 1 | 11 ± 1 | 24 ± 0 | 48 ± 3 | 1190 |
| podJΔ589-639 Δ921-974 | 6 ± 3 | 23 ± 3 | 23 ± 4 | 48 ± 4 | 1241 |
| DivK: | |||||
| Wild type | 0 ± 0 | 12 ± 9 | 56 ± 4 | 32 ± 11 | 887 |
| ΔpodJ | 0 ± 0 | 39 ± 14 | 20 ± 3 | 42 ± 17 | 1299 |
| podJΔ589-639 | 0 ± 0 | 33 ± 14 | 24 ± 21 | 43 ± 21 | 901 |
| podJΔ921-974 | 0 ± 0 | 18 ± 9 | 39 ± 14 | 43 ± 21 | 899 |
| podJΔ589-639 Δ921-974 | 0 ± 0 | 40 ± 11 | 23 ± 12 | 38 ± 12 | 818 |
| CckA: | |||||
| Wild-type | 0 ± 0 | 32 ± 9 | 49 ± 1 | 19 ± 9 | 898 |
| ΔpodJ | 0 ± 0 | 56 ± 11 | 22 ± 5 | 23 ± 7 | 818 |
| podJΔ589-639 | 0 ± 0 | 49 ± 10 | 26 ± 10 | 26 ± 12 | 672 |
| podJΔ921-974 | 0 ± 0 | 42 ± 13 | 31 ± 19 | 27 ± 11 | 739 |
| podJΔ589-639 Δ921-974 | 0 ± 0 | 49 ± 5 | 26 ± 9 | 25 ± 5 | 841 |
*
Percentages are derived from number of cells exhibiting indicated fluorescent localization out of total predivisional cells counted (including unlabeled cells). Numbers are averages and standard deviations based on three independent trials.