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. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: J Immunol. 2012 Apr 6;188(10):4951–4958. doi: 10.4049/jimmunol.1103160

FIGURE 3. NLRC5 cooperates with ATF1 and the transcriptional co-activators CBP/p300, and GCN5.

FIGURE 3

Transactivation of the HLA-B promoter by NLRC5 (A) or CIITA (B) with co-expression of ATF1 and the transcriptional co-activators CBP, p300 and GCN5 was examined. Tera-2 cells were transiently transfected with either empty control vector or the indicated expression plasmids and cells were harvested 48 hrs post transfection. Cell lysates were analyzed using the dual-luciferase assay and normalized against Renilla firefly activity. Data are a representative of several independent experiments performed in duplicates and are plotted as fold induction with respect to the control vector. Error bars represent ± SD. ns, not significant; *p < 0.05; **p < 0.01.