Skip to main content
. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Mol Microbiol. 2012 Apr 13;84(4):608–619. doi: 10.1111/j.1365-2958.2012.08046.x

Figure 6. PanM-dependent cleavage of pro-PanD.

Figure 6

Bands were visualized by Western blot analysis with rabbit polyclonal antibodies against PanD. A. Time course of the incubation of pro-PanD with PanM (top gel) or without PanM (bottom gel). B. Percent PanD cleavage as a function of time; PanD incubated alone (circles), or with PanM (triangles). Percentages were calculated by densitrometry analysis. The r2 for the PanD with PanM curve fit line was 0.87. C. Immunoprecipitation of PanD from S. enterica wildtype (lane 2) and ΔpanM strains (lane 3). Lane 1 contained PageRuler™ pre-stained protein ladder (Fermentas), with the 15 kDa standard indicated on the blot.