Skip to main content
. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: J Immunol. 2012 Apr 13;188(10):4866–4875. doi: 10.4049/jimmunol.1200402

Figure 6. TGFβ and CD103 expression is required for maintenance of IEL memory.

Figure 6

(A) C57Bl/6J mice were infected with LCMV Armstrong one day after transfer of a 1:1 ratio of WT P14 and CD103−/− P14. 4.5, 7, and 30 days later, the ratio of each population was assessed in the indicated tissues. (B–F) C57Bl/6J mice that received either WT Thy1.1+ P14 or CD103−/− Thy1.1+ P14 and infected with LCMV Armstrong were analyzed by immunohistochemistry 30 days later. (B) The indicated region of frozen small intestine `pinwheels' were assessed for SI IEL (C) and SI LPL (D, representative images, DAPI indicates nuclei, Thy1.1 indicates P14). (E) Number of WT or CD103−/− P14 counted in epithelium or lamina propria per 106 nucleated cells (approximately 1.5×105 nuclei were counted per mouse within the indicated region). n=3. (F) The ratio of SI IEL vs. LPL P14 that were counted in B–E. ***p<0.001, *p<0.05, Student's unpaired t test. Error bars indicated SEM.